Fine Structure of Changes Produced in Cultured Cells Sampled at Specified Intervals During a Single Growth Cycle of Polio Virus

Primary suspended cultures of rhesus monkey kidney cells were infected with poliomyelitis virus, type 1 (Brunhilde strain). The release of virus from these cells over a one-step growth curve was correlated with their change in fine structure, as seen in the electron microscope. Most of the cells were infected nearly simultaneously, and morphological changes developed in the cells were sufficiently synchronous to be classified into three stages. The earliest change (stage I) became visible at a time when virus release into the culture fluid begins, some 3 hours after adsorption. Accentuation of the abnormal characteristics soon occurs, at 4 to 7 hours after adsorption, and results in stage II. Stage III represents the appearance of cells after their rate of virus release had passed its maximum, and probably the abnormal morphology of these cells reflects non-specific physiological damage. There seems to be consistency between the previously described cellular changes as seen under the light microscope and the finer scale changes reported here. Cytoplasmic bodies, called U bodies, were seen in large number at the time when the virus release was the most rapid (stage II). While these bodies are not of proper size to be considered polio virus, they seem to be specifically related to the infection. No evidence was found for the presence of particles that could even be presumptively identified with those of polio virus

MOTIVATION IN WORK

Studying motivation and the motives that make people act in a certain way has a fundamental importance in the management of human resources. The whole human behaviour has a cause, which is a consequence of hereditary combination and development. At the basis of human behaviour there are the needs, wishes and motives. People try to fulfil certain objectives or purposes, which when reached will satisfy their needs. Of course, all the people in the organization participate in fulfilling them, each person with a different intensity. If we were to arrange the employees after their intensity with which they participate in fulfilling the companies’ objectives, then at the peak of the pyramid would be those who act with the most intensity to realize the objectives and at the bottom of the pyramid would be those who act with very little intensity. That is why managers of a company must identify motivational factors which make individuals who are at the bottom of the pyramid want to take more initiative to reach a higher performance level.motivation, behavior, human resources, performance, objectives

POSSIBILITIES OF INITIAL ESTIMATION AND FURTHER VALIDATION OF INSIDE CONTROL RISK

The approach option of the theme of this essay had in mind the distinguished importance of the objective of inside control regarding the segmentation with maximum exigency of the risks which can disadvantageously influence the fulfillment of the entity objectives. We considered essential the use of adequate procedures regarding the estimation and documentary validation of inside control risk. Regarding the initial estimation we analyzed the phases that are justified to be followed (four) and the proper steps which are followed (ten), starting with the assessment of audit objectives on types of operations and ending with reporting to the competent institutions. Regarding initial risk validation we examined the subsequent circumstances of this operation which are influencing the forecasted risk level, like: adjourning of control mechanisms applications, the apparition of new legal procedures, the alterations of the entity’s politics, etc.critical; inside control, initial risk estimation, subsequent risk validation, risk estimation procedure.

Ghetto of Venice: Access to the Target Node and the Random Target Access Time

Random walks defined on undirected graphs assign the absolute scores to all nodes based on the quality of path they provide for random walkers. In city space syntax, the notion of segregation acquires a statistical interpretation with respect to random walks. We analyze the spatial network of Venetian canals and detect its most segregated part which can be identified with canals adjacent to the Ghetto of Venice.Comment: 14 pages, 3 figure

EARNED VALUE MANAGEMENT.CASE STUDY USING MICROSOFT PROJECT

A formalism and a corresponding notation for earned value analysis are presented. With compact, consistent, mnemonic notation, earned value calculations become more transparent and flexible, leading to insights about standard quantities and advances through new measures. As an example of the notation’s utility, it is used to generate a modified earned value approach that weights quantities according to their position in a project’s timeline.earned value, managing projects, progress, cost, cash flow management

THE ROLE OF DATA ARCHITECTURE AS A PART OF ENTERPRISE ARCHITECTURE

In the early days of computing, technology simply automated manual processes with greater efficiency. The new organizational context provides input into the data architecture and is the primary tool for the management and sharing of enterprise data. It enables architects, data modelers, and stakeholders to identify, classify, and analyze information requirements across the enterprise, allowing the right priorities for data sharing initiatives. Data architecture states how data are persisted, managed, and utilized within an organization. Data architecture is made up of the structure of all corporate data and its relationships to itself and external systems. In far too many situations, the business community has to enlist the assistance of IT to retrieve information due to the community's inconsistency, lack of intuitiveness, or other factors. The goal of any architecture should illustrate how the components of the architecture will fit together and how the system will adapt and evolve over time.data architecture, enterprise architecture, business process planning,databases, business objects.

Laser wakefield and direct acceleration in the plasma bubble regime

Laser wakefield acceleration (LWFA) and direct laser acceleration (DLA) are two different kinds of laser plasma electron acceleration mechanisms. LWFA relies on the laser-driven plasma wave to accelerate electrons. The interaction of ultra-short ultra-intensive laser pulses with underdense plasma leads the LWFA into a highly nonlinear regime (“plasma bubble regime”) that attracts particular interest nowadays. DLA accelerates electrons by laser electromagnetic wave in the ion channel or the plasma bubble through the Betatron resonance. This dissertation presents a hybrid laser plasma electron acceleration mechanism. We investigate its features through particle-in-cell (PIC) simulations and the single particle model. The hybrid laser plasma electron acceleration is the merging concept between the LWFA and the DLA, so called laser wakefield and direct acceleration (LWDA). The requirements of the initial conditions of the electron to undergo the LWDA are determined. The electron must have a large initial transverse energy. Two electron injection mechanisms that are suitable for the LWDA, density bump injection and ionization induced injection, are studied in detail. The features of electron beam phase space and electron dynamics are explored. Electron beam phase space appears several unique features such as spatially separated two groups, the correlation between the transverse energy and the relativistic factor and the double-peak spectrum. Electrons are synergistically accelerated by the wakefield as well as by the laser electromagnetic field in the laser-driven plasma bubble. LWDA are also investigated in the moderate power regime (10 TW) in regarding the effects of laser color and polarization. It is found that the frequency upshift laser pulse has better performance on avoiding time-jitter of electron energy spectra, electron final energy and electron charge yield. Some basic characters that related to the LWDA such as the effects of the subluminal laser wave, the effects of the longitudinal accelerating field, the electron beam emittance, the electron charge yield and potentially applications as radiation source are discussed.Physic